Methods

Overview

  • histology, immunohistochemistry, and tracer backfills
  • imaging techniques and 3D reconstruction
  • electrophysiology
  • behavioral experiments in the field and in the lab, bioessays

Sectioning devices

  • vibratome Zeiss Hyrax V-50
  • rotary microtome Leica RM 2145
  • rotary microtome Microm HM 360
  • sliding microtome Leica SM2000 R
[top row left to right] Birgus brain (silver impregnation), Coenobita olfactory system (Azan stain and silver impregnation), Penaeus visual system (Azan stain), Rimicaris brain (immunohistochemistry), Stenopus antennae (bright-field micrograph); [bottom row left to right] Nebalia brain (S-phase marker and immunohistochemistry), Endeis nervous system (reconstruction), Procambarus caudal papilla (in situ hybridization and immunohistochemistry), cockroach nymph (µCT), cockroach nymphs in amber (macrophotography)

Optical equipment

(see also Imaging Center)

  • macro- and microphotographic setup
  • fluorescence microscope with co-observation module Nikon SMZ800N
  • epifluorescence microscope Nikon Eclipse 90i
  • light microscope Zeiss Axiophot
  • scanning electron microscope Zeiss EVO LS10
  • transmission electron microscope Jeol JEM 1011
  • confocal laser scanning microscope Leica SP5 II
  • X-ray micro-computed tomograph Zeiss Xradia XCT-200

Visualisation software and 3D printing

  • Bitplane Imaris
  • FEI Amira
  • Ultimaker 2 (3D Printer)