Dr. Jakob Krieger

Zoologisches Institut und Museum
Cytologie und Evolutionsbiologie

Soldmannstr. 23
Haus 6.1
17489 Greifswald

Tel.: +49 (0)3834 420 4109
Fax: +49 (0)3834 420 4067
jakob.kriegeruni-greifswaldde


Research interests

The conquest of land occurred at least five times convergently within the Crustacea. We are interested in the terrestrial adaptations of the sensory organs and the brain of various terrestrial crustacean taxa in contrast to their nearest marine relatives. In this framework, our main focus lies on the morphology of the central olfactory processing areas.

For our research, we use classical histological, light microscopic techniques, X-Ray computed tomography as well as backfills with neuronal tracers, immunofluorescence labeling in combination with confocal laser-scan microscopy and 3D-reconstruction. In addition to the morphological data, our aim is to study the capacities for orientation of e.g. the giant robber crab Birgus latro in field experiments with the aid of behavioral tests as well as radio- and satellite telemetry. In addition to studies on robber crabs, we use different species of Coenobita as comparison model for bioassays in windtunnel experiments focussing on olfactory behavior. These experimental and morphological data will improve our knowledge of terrestrial adaptations and the evolution of crustacean sensory and nervous systems.

For studying the cognitive capabilities of hermit crabs, we conduct behavioral two-choice experiments on Coenobia compressus using 3D-printed gastropod shells in the lab as well as in the field (Peninsula Osa, Costa Rica).

Research methods:

  1. Neuroanatomical methods (Histology, Immunohistochemistry, X-Ray computed computertomography, Microscopy (light and confocal laser-scanning microscopy) and 3D reconstruction using AMIRA and IMARIS)
  2. Telemetric methods (GPS analyses using ArcGIS, 3D accelometry)
  3. Behavioral bioassays (camera tracked bioassays in windtunnels, motion tracking analysis using OSP Tracker, field experiments with 3D-printed shells)
left: Birgus latro (Anomura): whole animal and brain right: Gecarcoidea natalis (Brachyura): whole animal and brain (fotos of whole animals by S. Harzsch and B. S. Hansson on Christmas Island, December 2008; fluorescence microscopical fotos of brains by J. Krieger, 2009)
Three dimensional brain reconstruction of Birgus latro
Summary figure for telemetric studies on Christmas Island from 2008 to 2011 shows an animal carrying a GPS transmitter and typical movement patterns (blue and pink paths) out of all waypoints (black dots on background map).
Curriculum Vitae
Awards and Grants
3rd Place - Students Poster Award of the 3rd International Congress on Invertebrate Morphology (ICIM3)
3rd Place - Poster Award of the 19th Conference of German Crustaceologists (CrustiTag19)
500 € Travel grant of the Glaxo-Smith-Kline-Foundation
875 € Travel grant of the German Academic Exchange Service (DAAD)
250 € Travel grant of the University of Greifswald
Poster ICIM3 Berlin 2014
Memberships
Graduiertenakademie der Universität Greifswald
Deutsche Zoologische Gesellschaft (DZG)
International Society for Invertebrate Morphology (ISIM)
Peer Review and Editorial Activity
Peer Review for Zoologischer Anzeiger - A Journal of Comparative Zoology (Elsevier); Crustaceana (Brill)
Guest Editorial for Arthropod, Structure & Development (Elsevier)
Field trips and collecting journeys
2018 and 2019 Field trip to Osa Peninsula, Costa Rica for studying shell-choice behavior of C. compressus using 3D-printed shells
2017 Short research stay at Dartmouth College, Hanover (NH; USA) at the lab of Prof. Mark Laidre
2017 Field trip to Osa Peninsula, Costa Rica for studying odor-guided behavior of Coenobita compressus
2011 - 2012 GPS-telemetric and behavioral studies on Birgus latro on Christmas Island, Indian Ocean, Australia
2010 Collecting journey of the Zoological Institute of the University of Greifswald to Christmas Island, Indian Ocean, Australia
2009 Collecting journey of the Zoological Institute of the University of Greifswald to Roscoff, France
2008 Wadden Sea Excursion on Sylt (Germany) of the University of Rostock
2008 Zoological field trip of the University of Rostock to Ibiza, Spain
2008 Two ichthyological boat trips on the Baltic with the FFS "Solea" of the Johann-Heinrich-von-Thünen-Institut of Rostock
2007 Zoological field trip of the University of Rostock to Ibiza, Spain

Publications

2019

  • Krieger J & Spitzner F (accepted). X-ray microscopy of the larval crustacean brain. In: S. Sprecher (ed.) Brain Development Methods and Protocols, Springer.

2018

  • Nischik ES & Krieger J (2018) Evaluation of standard imaging techniques and volumetric preservation of nervous tissue in genetically identical offspring of the crayfish Procambarus fallax cf. virginalis (Marmorkrebs). PeerJ 6:e5181 Open Access
  • Harzsch S & Krieger J (2018) Crustacean olfactory systems: a comparative review and a crustacean perspective on insect olfactory systems. Progress in Neurobiology 161:23-60. Download via scienceirect.com.

2016

  • Holst S, Michalik P, Noske M, Krieger J, Sötje S (2016) Potential of X-ray micro-computed tomography for soft-bodied and gelatinous cnidarians with special emphasis on scyphozoan and cubozoan statoliths. Journal of Plankton Research 38 (5): 1225-1242. Download via Oxford Journals.
  • Krieger J (2016) Neuroanatomische und neuroethologische Untersuchungen am größten terrestrischen Gliederfüßer, dem Palmendieb Birgus latro (Linnaeus, 1767) (Crustacea, Anomala), auf der Weihnachtsinsel (Indischer Ozean). Inaugural-Dissertation Universität Greifswald. 157 pp. pdf via OPUS-Datenbank der Universitätsbibliothek Greifswald.
  • Krieger J, Drew MM, Hansson BS, Harzsch S (2016) Notes on the foraging strategies of the Giant Robber Crab Birgus latro (Anomala) on Christmas Island: Evidence for active predation on Red Crabs Gecarcoidea natalis (Brachyura). Zoological Studies 55:6. Open Access. Download additional movie file (.mp4) by using right-click on this link and "save target as...".

2015

  • Krieger J*, Braun P*, Rivera NT, Schubart CD, Müller CHG, Harzsch S (2015) Comparative analyses of olfactory systems in terrestrial crabs (Brachyura): Evidence for aerial olfaction?. PeerJ 3: e1433. *shared first authorshipOpen Access
  • Harzsch S, Krieger J, Faulkes Z (2015) Astacida. In "Evolutionary Developmental Biology of Invertebrates 4: Ecdysozoa II: Crustacea" A. Wanniger (ed.), Springer. Buy chapter or book via www.springer.com

2012

  • Krieger J, Grandy R, Drew MM, Erland S, Stensmyr MC, Harzsch S, Hansson BS (2012) Giant robber crabs monitored from space: GPS-based telemetric studies on Christmas Island (Indian Ocean). PLoS ONE 7(11): e49809. Open Access
  • Krieger J, Sombke A, Seefluth F, Kenning M, Hansson BS, Harzsch S (2012) Comparative brain architecture of the European shore Crab Carcinus maenas (Brachyura) and the Common hermit Crab Pagurus bernhardus (Anomura). Cell and Tissue Research. 348(1):47-69. Download via www.springerlink.com

2011

  • Harzsch S, Rieger V, Krieger J, Seefluth F, Strausfeld NJ, Hansson BS (2011) Transition from marine to terrestrial ecologies: Changes in olfactory and tritocerebral neuropils in land-living isopods. Arthropod Structure and Development 40: 244-257. Download via www.sciencedirect.com

2010

  • Krieger J, Sandeman RE, Sandeman DC, Hansson BS, Harzsch S. (2010) Brain architecture of the largest living land arthropod, the Giant Robber Crab Birgus latro (Crustacea, Anomura, Coenobitidae): evidence for a prominent central olfactory pathway? Frontiers in Zoology. 7:25. Open Access
Talks
  • Krieger J, Hörnig MK, Laidre ME (2019)Verhaltensversuche im Maßanzug – Schalenwahl als Grundlage für Kognitionsexperimente am Pazifischen Landeinsiedler Coenobita compressus H. Milne-Edwards, 1836. CrustiTag 2019, Munich, Germany. Travel grant of the University of Greifswald of 250 €
  • Krieger J (2018) Hermit housing situation in Costa Rica - Shell choice experiments and olfactory guided behavior of the Pacific hermit crab Coenobita compressus H. Milne-Edwards, 1836 (Malacostraca, Anomala). Institute Symposium of the Zoological Institute and Museum of the University of Greifswald, Germany.
  • Krieger J (25.04.2018) Neuroethology of terrestrial crustaceans. University of Ulm, Germany. Invited talk
  • López AI, Haug JT, Hörnig MK, Krieger J, Haug C (2017) Convergent evolution of raptorial appendages in arthropods: first steps towards a quantitative approach. 110th Annual Meeting of the German Zoological Society, Bielefeld, Germany.
  • Krieger J, Sombke A, Harzsch S (2017) Three somatotopic maps for one sensorial modality – Brain architecture of the banded cleaner shrimp Stenopus hispidus (Olivier, 1811); (Malacostraca, Stenopodidea). 4th International Congress on Invertebrate Morphology (ICIM4) Moscow, Russion Federation Travel grant of the German Academic Exchange Service (DAAD) of 875 €
  • Krieger J, Winter M, Sombke A, Harzsch S (2016) Brain anatomy in the banded cleaner shrimp (Malacostraca, Stenopodidea) – comparison of mechanosensory neuropils in Stenopus hispidus and in other malacostracan taxa. 4th Symposium of the Arthropod Neuroscience Network, Kiel, Germany
  • Kirchhoff T, Krieger J, Harzsch S (2014) Vom Wasser an Land: Einblicke in die Postembryonalentwicklung des Palmendiebes Birgus latro (Linnaeus, 1767); 7. Graduiertentreffen Fachgruppe Morphologie der DZG, Hamburg, Germany.
  • Krieger J, Hansson BS, Harzsch S (2014) Crabs on the move - Telemetric analyses of migrations and daily activity of the giant robber crab Birgus latro on Christmas Island (Indian Ocean). 8th International Crustacean Congress, Frankfurt am Main, Germany.
  • Harzsch S, Krieger J, Hansson BS (2014) Terrestrial adaptations of the sense of smell in terrestrial Anomura and Brachyura. 8th International Crustacean Congress, Frankfurt am Main, Germany.
  • Krieger J, Braun P, Harzsch S (2014) Evolution of the central olfactory pathway in terrestrial brachyurans (true crabs) versus terrestrial anomurans (hermit crabs). Rauischholzhausen Seminar: Development and Plasticity of the insect nervous system, Rauischholzhausen, Germany.
  • Krieger J, Grandy R, Drew MM, Erland S, Stensmyr MC, Harzsch S, Hansson BS (2012) Giant Robber Crabs (Birgus latro) monitored from space GPS-based telemetric studies on Christmas Island. 105. Jahrestagung der Deutschen Zoologischen Gesellschaft, Konstanz, Germany.
  • Krieger J, Harzsch S and Hansson BS (2011) GPS– und Radiotelemetrische Untersuchungen zur Migration des Palmendiebes, Birgus latro Linnaeus, 1767 (Crustacea, Decapoda, Anomura) auf der Weihnachtsinsel. 15. Crustaceologen-Tagung, Regensburg, Germany.
  • Krieger J, Sandeman RE, Sandeman DC, Hansson BS and Harzsch S (2010) Brain architecture of the largest living land arthropod, the Giant Robber Crab Birgus latro (Crustacea, Anomura, Coenobitidae): evidence for a prominent central olfactory pathway? EVOLF MMX Conference, Christmas Island, Australia.
  • Krieger J, Seefluth F, Sandeman DC, Sandeman RE, Hansson BS and Harzsch S (2010) Brain architecture of Birgus latro L., 1767 (Crustacea, Decapoda, Anomura) in comparison to other terrestrial and marine decapod crustaceans. 103. Jahrestagung der DZG, Hamburg, Germany.
  • Krieger J (2010) Die Gehirnarchitektur von Birgus latro (Linnaeus, 1767), (Crustacea, Decapoda, Anomura) Institutssymposium Zoologisches Institut Universität Greifswald, Germany.
  • Krieger J, Seefluth F, Sandemann DC, Hansson BS und Harzsch S (2009) Die Gehirnarchitektur von Birgus latro (Anomura) und Gecarcoidea natalis (Brachyura): Beispiele für terrestrische Adaptionen des Geruchssinns. 2. Graduiertenforum Fachgruppe Morphologie der DZG, Greifswald, Germany.
Poster
  • Hörnig MK, Harzsch S, Krieger J (2019) Smarte Reinigungskräfte - Das Gehirn und sensorische Kopfstrukturen der gebänderten Scherengarnele Stenopus hispidus (Olivier, 1811). 19th Conference of German Crustaceologists (CrustiTag19), München 3rd place Poster Award
  • Hörnig MK, Rath A-C, Martin I, Harzsch S, Krieger J (2018) Sensing the environment – an atlas of the malacostracan sensory appendages. 111th Annual Meeting of the German Zoological Society, Greifswald.
  • Krieger J, Laidre ME, Hörnig, MK (2018) Shell-use as a tool in the Pacific hermit crab Coenobita compressus H. Milne-Edwards, 1836, 111th Annual Meeting of the German Zoological Society, Greifswald.
  • López AI, Haug JT, Hörnig MK, Krieger J, Haug, C (2017) Convergent evolution of raptorial appendages in arthropods: first steps towards a quantitative approach. 110th Annual Meeting of the German Zoological Society, Bielefeld, Germany
  • Richter AC, Hörnig MK, Martin I, Harzsch S, Kieger J (2017). Sensing the environment – the morphological diversity of first and second antennal appendages in Malacostraca. 18. Crustaceologen-Tagung, Berlin
  • Hörnig MK, López AI, Krieger J, Haug JT, Haug C (2017). Konvergente Evolution der Raubbeine bei Gespenstkrebsen und Gottesanbeterinnen: ein funktionsmorphologischer Vergleich. 18. Crustaceologen-Tagung, Berlin
  • Kreifelts V and Krieger J (2017) A crustacean invader’s brain - the anatomy of the central nervous system in the Japanese skeleton shrimp Caprella mutica (Malacostraca, Amphipoda), 110th Annual Meeting of the German Zoological Society, Bielefeld, Germany.
  • Haug C, López AI, Hörnig MK, Krieger J, Haug JT (2017) Convergent evolution of raptorial appendages in arthropods. 4th International Congress on Invertebrate Morphology (ICIM4), Moscow, Russion Federation.
  • Kreifelts V and Krieger J (2016) Brain anatomy in the Japanese skeleton shrimp Caprella mutica (Malacostraca, Amphipoda); 109th Annual Meeting of the German Zoological Society, Kiel, Germany.
  • Krieger J, Winter M, Sombke A, Harzsch S (2016) Neuroanatomy in the banded cleaner shrimp (Malacostraca, Stenopodidea) – Structure of mechanosensory neuropils in Stenopus hispidus (Olivier, 1811); 109th Annual Meeting of the German Zoological Society, Kiel, Germany.
  • Krieger J, Hansson BS, Harzsch S (2015) Evolution of terrestrial olfaction in representatives of hermit crabs (Anomala) with notes on true crabs (Brachyura); 14th Europoean Symposium for Insect Taste and Olfaction (ESITO), Villasimius (Sardinia), Italy. Travel grant of the GSK Foundation of 500 €
  • Rudolf, N.R., Haug, C., Harzsch, S., Krieger, J., Hamasaki, K., Haug, J.T. (2015). Die Metamorphose des Palmendiebes Birgus latro (Decapoda, Coenobitidae). Crustaceologentagung 2015, Bremerhaven.
  • Krieger J, Kirchhoff T, Hamasaki K, Harzsch S (2014) Transition to land - the larval development of the giant robber crab Birgus latro (Linnaeus, 1767), with a focus on antennal and brain morphology; 3rd International Congress on Invertebrate Morphology, Berlin, Germany. 3rd place Students Poster Award
  • Braun P, Krieger J, Hansson BS, Harzsch S (2014) Structure of the central olfactory pathway in marine versus terrestrial brachyuran crustaceans; 3rd International Congress on Invertebrate Morphology, Berlin, Germany.
  • Krieger J, Grandy R, Drew MM, Erland S, Stensmyr MC, Harzsch S, Hansson BS (2013) Telemetric studies on Birgus latro on Christmas Island; 16. Crustaceologen-Tagung, Greifswald, Germany.
  • Krieger J, Grandy R, Drew MM, Erland S, Stensmyr MC, Harzsch S, Hansson BS (2012) Giant Robber Crabs (Birgus latro) monitored from space: GPS-based telemetric studies on Christmas Island (Indian Ocean); 14th ISBE, Lund, Sweden.
  • Krieger J, Seefluth F, Sandeman RE, Sandeman DC, Hansson BS and Harzsch S (2011) Terrestrial adaptations of olfactory systems – A comparative neuroanatomical study of terrestrial and marine members of the Meiura; 9th Göttingen Meeting of the German Neuroscience Society, Germany / The 9th International Conference and Workshop on Lobster Biology and Management, Bergen, Norway.
  • Harzsch S, Krieger J, Rieger V, Strausfeld NJ, Hansson BS (2011) Transition from marine to terrestrial ecologies: changes in olfactory and tritocerebral neuropils in land-living isopods; 15. Crustaceologen-Tagung, Regensburg, Germany.
  • Seefluth F, Krieger J, Harzsch S (2010) Brain architecture of the European shore crab Carcinus maenas (Linnaeus, 1758); 103. Jahrestagung der DZG, Hamburg, Germany.
  • Seefluth F, Krieger J, Harzsch S (2009) Die Gehirnarchitektur der gemeinen Strandkrabbe (Carcinus maenas). 2. Graduiertenforum Fachgruppe Morphologie der DZG, Greifswald, Germany.